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谷氨酸是哺乳类中枢神经系统的一种重要的神经递质。为了探讨谷氨酸是否作用于延髓网状背核的神经元 ,采用免疫组织化学方法 ,对代谢型谷氨酸受体的 7种亚型 ( m Glu R1-7)以及离子型谷氨酸受体中的 N-甲基 -天门冬氨酸 1型受体 ( NM-DAR1)在延髓网状背侧亚核内分布的情况进行了研究。结果显示 ,几种谷氨酸受体的免疫阳性产物主要定位在神经元的胞体和树突上。在延髓网状背侧亚核可以观察到 m Glu R5、m Glu R7和 NMDAR1这 3种受体亚型的免疫阳性神经元 ,其中 m Glu R7免疫阳性结构主要为终末样结构 ,而 NMDAR1免疫阳性结构主要定位于神经元胞体。在该核内未见 m Glu R2 -3、m Glu R4以及 m Glu R6等受体亚型的免疫阳性神经元。研究结果表明 ,延髓网状背侧亚核是谷氨酸的作用部位 ,并提示谷氨酸与该核的功能调节有关
Glutamate is an important neurotransmitter in the mammalian central nervous system. In order to investigate whether glutamate acts on the neurons of the medulla oblongata and dorsal horn, seven subtypes of metabotropic glutamate receptors (m Glu R1-7) and the ionic glutamate receptor The distribution of N-methyl-aspartate type 1 receptor (NM-DAR1) in the subnucleus reticularis dorsalis was studied. The results showed that several immunoreactive products of glutamate receptors were mainly localized on the soma and dendrites of neurons. Immunoreactive neurons of the three receptor subtypes, m Glu R5, m Glu R7 and NMDAR1, were observed in the subnucleus of the medulla oblongata. The immunopositive structure of m Glu R7 was mainly terminal-like, whereas the immunization of NMDAR1 The positive structure is mainly located in the neuronal soma. No immunoreactive neurons of receptor subtypes m Glu R2 -3, m Glu R4, and m Glu R6 were found in the nucleus. The results show that the medulla oblongata dorsal submucosa is the role of glutamate sites, suggesting that glutamate and the nuclear regulatory function