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目的了解杭州市家蝇抗药性水平,为杀虫剂的合理使用提供科学依据。方法采用微量点滴法检测杭州市家蝇现场种群对溴氰菊酯、高效氯氰菊酯、二氯苯醚菊酯、EBT、敌敌畏、胺菊酯6种杀虫剂抗药性水平。结果6种杀虫剂对家蝇现场种群的半数致死剂量(LD50),其化学毒性强度由低到高依次为二氯苯醚菊酯、溴氰菊酯、胺菊酯、EBT、高效氯氰菊酯、敌敌畏。与敏感品系比较,溴氰菊酯抗性水平提高最多,抗性系数为338.00(LD50为0.1014μg/只);其次为高效氯氰菊酯,抗性系数为86.77(LD50为0.7983μg/只);其他杀虫剂抗性水平接近或低于敏感品系,抗性系数在0.19～1.92。结论杭州市家蝇现场种群对溴氰菊酯、高效氯氰菊酯的抗性水平较高,敌敌畏等抗性呈升高趋势,在灭蝇工作中应注意避免大量单独使用。
Objective To understand the resistance level of housefly in Hangzhou and provide a scientific basis for the rational use of insecticides. Methods The micro-drip method was used to detect the resistance level of six insecticides to housefly in Hangzhou, including deltamethrin, beta-cypermethrin, permethrin, EBT, dichlorvos and tetramethrin. Results The LD50 of six kinds of insecticides on the field population of housefly were as follows: permethrin, deltamethrin, tetramethrin, EBT, beta-cypermethrin, Dichlorvos. Compared with the susceptible lines, deltamethrin showed the highest level of resistance, the resistance coefficient was 338.00 (LD50 was 0.1014μg / only), followed by beta-cypermethrin, the resistance coefficient was 86.77 (LD50 was 0.7983μg / Insecticide resistance levels were close to or lower than those of susceptible strains, with resistance coefficients ranging from 0.19 to 1.92. Conclusion The resistance of deltamethrin and beta-cypermethrin to housefly populations in Hangzhou City is high, and the resistance to dichlorvos and so on is on the rise. A large number of single-use antifungal agents should be avoided during the fly-killing operation.