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目的:建立妇炎平胶囊中苦参、苦参碱、蛇床子、蛇床子素、苦木的薄层色谱(TLC)鉴别方法以及HPLC外标法测定蛇床子素的含量。方法:采用TURNER KromalilC18柱(200mm×4.6mm;5μm)流动相为乙腈-水(65∶35),检测波长322nm;经TLC阴性对照和阳性对照试验,确认方中苦参、苦木、蛇床子成分,并经提取后HPLC外标法测定蛇床子素含量。结果:蛇床子素对照品在0.002978~2.978μg/mL范围内呈良好的线性关系,平均加样回收率为97.38%,RSD=2.20%(n=6);妇炎平胶囊中蛇床子素含量为3.482mg/g。结论:该方法操作简便易行、分离效果好,适用于妇炎平胶囊质量控制。
OBJECTIVE: To establish thin layer chromatography (TLC) identification method of Sophora flavescens, matrine, Cnidium, Osthole, Fructus Moss and Sophora flavescens in Fuyanying Capsule, and determine the content of Osthole in HPLC by external standard method. Methods: TURNER Kromalil C18 column (200mm × 4.6mm; 5μm) was used. The mobile phase was acetonitrile-water (65:35) and the detection wavelength was at 322nm. Components, and extracted by HPLC external standard method for determination of osthole content. Results: The osthole was linear in the range of 0.002978 ~ 2.978μg / mL with the average recovery of 97.38% and RSD = 2.20% (n = 6). The osthole content of Fu Yan Ping capsule 3.482 mg / g. Conclusion: The method is simple and easy to operate with good separation effect and is suitable for the quality control of Fuyanping capsule.