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以耐高温酿酒酵母为对象,研究高温酿酒酵母利用玉米秸秆为原料同步糖化产氢乙醇的影响因素,采用Plackett-Burman(PB)法对影响乙醇产率的5个因素进行筛选,采用CCD模型及响应面分析对影响因素进行优化设计,确定高温酿酒酵母利用玉米秸秆为原料同步糖化产氢乙醇的最佳工艺条件。研究结果表明:高温酿酒酵母利用玉米秸秆酶解液同步糖化产乙醇过程中接种量、温度、酶浓度和发酵时间4个因素对乙醇产量的影响最为明显,温度和发酵时间的交互作用最为显著。利用优化设计得到的最佳产乙醇工艺为:接种量7.4%、温度34.2℃、初始p H值5.0、酶浓度49.36 U/g。在此条件下,发酵105.12 h后乙醇产率可达59.88%,实验结果与响应面拟合方程的预测值(60.85%)吻合良好。
To study the influencing factors of simultaneous saccharification and ethanol production in high temperature Saccharomyces cerevisiae using corn stalks as raw materials, five factors influencing ethanol yield were screened by Plackett-Burman (PB) Response surface analysis of the influencing factors to optimize the design to determine the temperature of Saccharomyces cerevisiae using corn stalks as raw material for simultaneous saccharification hydrogen production of ethanol the best processing conditions. The results showed that the effects of inoculation amount, temperature, enzyme concentration and fermentation time on the yield of ethanol were the most significant among the Saccharomyces cerevisiae during the simultaneous saccharification of ethanol by using corn stalk hydrolyzate. The interaction between temperature and fermentation time was the most significant. The optimal ethanol production process was: inoculation amount of 7.4%, temperature of 34.2 ℃, initial pH value of 5.0 and enzyme concentration of 49.36 U / g. Under this condition, the yield of ethanol reached 59.88% after 105.12 h of fermentation. The experimental results were in good agreement with the predicted value of response surface fitting equation (60.85%).