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为获取人前列腺癌诱导基因家族新成员 ,以人前列腺癌细胞系PC 3的总RNA为模板 ,用RT PCR方法扩增人前列腺癌诱导基因 1(PTI 1) ,将其亚克隆至克隆载体pUC19,进行测序 .将克隆得到的PTI 1基因片段与国外报道的人PTI 1基因编码区cDNA同源性为 99.3% ,但第 72 6、74 6、116 7、12 70、132 6、144 9、16 94和 16 95位碱基分别由A、A、C、C、A、A、T和C替代了文献中的C、C、G、G、G、C、C和T .其中 6个碱基的不同 ,导致了第 36、183、2 17、2 36、2 77和 35 9位密码子代表的氨基酸分别由Lys替代Gln ,Arg替代Gly ,Ala替代Gly ,Ser替代Gly ,Thr替代Pro和Arg替代Cys .将所获得的基因在GenBank登录 ,登录号为AF3974 0 3.结果提示 ,获得了PTI 1家族新成员 ,并且与EF 1α分子的同源性较已报道的PTI 1高 .
To obtain a new member of the human prostate cancer-induced gene family, the human prostate cancer cell-inducing gene 1 (PTI 1) was amplified by RT-PCR using the total RNA of the human prostate cancer cell line PC 3 as a template and subcloned into the cloning vector pUC19 , And sequenced.The homology of the cloned PTI 1 gene with the coding region of human PTI 1 gene reported in foreign countries was 99.3%, but the 726,74 6,116 7,12 70,132 6,144 9, 16, 9 and 16 95 bases replaced C, C, G, G, C, C and T in the literature by A, A, C, C, A, A, T and C. Six of them The different amino acids of the 36th, 38th, the 2nd, the 2nd, the 2nd, the 2nd, the 2nd, the 2nd, the 2nd, the 2nd, 36th, 36th, 77th and 35th codons represent the amino acids Lys instead of Gln, Arg for Gly, Ala for Gly, Ser for Gly and Thr for Pro and Arg for Cys.The obtained gene was registered in GenBank under accession number AF3974 0 3. The results suggest that a new member of PTI 1 family was obtained and its homology with EF 1α was higher than that reported for PTI 1.