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从大亚湾野生群体选择亲本繁育1个养殖群体,受精第6天、23天与80天分别进行优选,按50%选留比率选留快生长个体,分别在受精后第3天、30天120天取样,利用8对SSR标记分析养殖群体遗传多样性与估计有效亲本数量。结果表明,在3个生长阶段每个位点的平均等位基因数分别为3.750、3.875和3.625,平均观察杂合度分别为0.324 2、0.527 3和0.372 4,平均期望杂合度分别为0.551 1、0.561 3和0.504 8。有效亲本数量的估计分别为38、28和30,3个生长阶段估算的有效亲本数量均比实际所用到的亲本数量少。选优管养措施导致养殖群体的遗传多样性与有效亲本数量降低。
One breeding population was selected from the wild population of Daya Bay to breed one breeding group. The 6th, 23th and 80th days of fertilization were optimized respectively. The fast growing individuals were selected according to the selection rate of 50%. After 3 days, 30 days and 120 days respectively Sampling, 8 pairs of SSR markers were used to analyze the genetic diversity of farmed population and the estimated number of effective parents. The results showed that the average number of alleles per locus at three growth stages were 3.750, 3.875 and 3.625, respectively. The average observed heterozygosities were 0.324 2, 0.527 3 and 0.372 4 respectively. The average expected heterozygosities were 0.551 1, 0.561 3 and 0.504 8. The estimated number of valid parents is 38, 28 and 30, respectively, and the estimated number of valid parents in the three growth stages is less than the number of parents actually used. The optimal control measures led to the decrease of genetic diversity and effective parents in cultured population.