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高速逆流色谱(HSCCC)是一项无固体载体即一种连续高效的新型液-液分配色谱技术,主要利用化合物在溶剂系统中分配系数的差异达到分离目的,在化合物分离以及单体制备等方面有广泛运用。综述了近年来高速逆流色谱技术在分离纯化黄酮苷元和黄酮苷类化合物中的应用,包括高速逆流色谱技术分离纯化已知和未知天然产物黄酮类成分、筛选黄酮活性成分,为今后高速逆流色谱在分离黄酮类化合物中的应用提供借鉴。
High-speed countercurrent chromatography (HSCCC) is a solid-free carrier that is a continuous and efficient new liquid-liquid partition chromatography technology, which mainly uses the difference of the partition coefficient of the compounds in the solvent system to achieve the separation purpose. In the aspects of compound separation and monomer preparation Widely used. In recent years, the applications of high-speed countercurrent chromatography in the purification and purification of flavonoid aglycone and flavonoid glycosides were summarized. High-speed countercurrent chromatography was used to separate and purify flavonoids of known and unknown natural products, and to screen the active ingredients of flavonoids for the future high-speed countercurrent chromatography In the separation of flavonoids in the application of reference.