目的:采用高效液相色谱法建立细圆藤药材的指纹图谱,对多批细圆藤药材进行质量评价,为细圆藤药材质量标准的研究提供一定的科学依据。方法:采用高效液相色谱法,Waters XBridge C18色谱柱(4.6 mm×250 mm,5μm),以乙腈-0.2%磷酸为流动相梯度洗脱,流速1.0 m L·min-1,检测波长327 nm,柱温30℃;建立细圆藤药材HPLC指纹图谱,利用“中药色谱指纹图谱相似度评价系统”2012.1版软件对10批药材进行相似度评价,并利用SPSS 22.0软件对所得10批细圆藤药材色谱数据采用Ward法,平方Euclidean距离度量标准进行聚类分析,并进行主成分分析。结果:以S4号样品的图谱为参照图谱,采用多点校正和Mark峰匹配,10批不同产地的细圆藤药材共标定了11个共有峰,并用对照品指认出绿原酸;10批样品指纹图谱的相似度均>0.9,通过聚类及主成分分析,将10批不同产地的药材分为4类。结论:该方法具有较高的稳定性,方法简便且专属性强,所建立的指纹图谱具有较好的稳定性,对进一步研究细圆藤药效物质基础有重要意义,也为广西壮药细圆藤的质量控制与评价提供了参考。 OBJECTIVE: To establish the fingerprint of Salvia elatiorrhiza with HPLC and to evaluate the quality of Salvia elata. To provide a scientific basis for the study of the quality standard of Salvia formosa. Methods: The mobile phase was eluted with a mobile phase of acetonitrile-0.2% phosphoric acid at a flow rate of 1.0 m L · min-1 on a Waters XBridge C18 column (4.6 mm × 250 mm, 5 μm) using high performance liquid chromatography , The column temperature was 30 ℃. The HPLC fingerprinting of Artemisia annua was established. The similarity of 10 batches of medicinal materials was evaluated by using the software of “Similarity Evaluation System of Chromatographic Fingerprinting of Traditional Chinese Medicine”, and the software was analyzed by SPSS 22.0 software Radix et Rhizoma Radix et Rhizoma Radix et Rhizoma Radix et Rhizoma Radix et Rhizoma Radix et Rhizoma Radix et Rhizoma Radix et Rhizoma Radix et Rhizoma Rhei were analyzed by Ward method, square Euclidean distance measurement standard and principal component analysis. Results: Based on the map of S4 sample, multi-point calibration and Mark peak matching were used. Ten common peaks were identified in 10 batches of medicinal materials from different origins, and chlorogenic acid was identified with the reference substance. Ten batches of samples The similarity of fingerprints was> 0.9. According to the cluster analysis and principal component analysis, 10 batches of medicinal materials from different areas were divided into 4 categories. Conclusion: The method has high stability, the method is simple and proprietary, and the established fingerprint has good stability, which is of great significance for further study on the substance basis of Pharmacordia sp Round vine quality control and evaluation provides a reference.