论文部分内容阅读
目的 为探讨泡状棘球蚴病宿主体内淋巴细胞在免疫调节和发病中的作用。方法 对泡球蚴感染BALB/c小鼠观察至 2 5周 ,在不同时间取脾制备细胞悬液 ,检测CD+ 4 ,CD+ 8细胞数量。对 2 5例泡球蚴病患者和 18例健康人群 ,用FCM分析了CD+ 3 ,CD+ 4 ,CD+ 8,CD+ 19,CD+ 3 8,CD+ 56和HLA -DR+ 细胞的变化。结果 泡球蚴感染BALB/c小鼠后 ,1～ 8周以CD+ 4 细胞为主 ,随后CD+ 4 细胞减少 ,CD+ 8细胞增加 ,2 0周后改变显著 (P <0 0 5 ) ,CD+ 4 /CD+ 8比值迅速倒置。泡状棘球蚴病患者CD+ 3细胞未发生改变 ,CD+ 4 细胞较正常对照组下降 (P <0 0 5 ) ,CD+ 8细胞上升 (P <0 0 5 ) ,使CD+ 4 /CD+ 8比值降低 (P <0 0 5 )。CD+ 56细胞较正常对照组显著性降低 (P <0 0 1) ,CD+ 19,CD+ 3 8和HLA -DR+ 细胞未发生改变 (P >0 0 5 )。结论 泡球蚴感染小鼠前 8周 ,以CD+ 4 细胞反应为主 ,具有保护性免疫。感染后期逐渐以CD+ 8细胞为主 ,使机体呈免疫抑制状态 ,有利于泡球蚴生存。泡状棘球蚴病患者机体呈免疫抑制状态 ,有利于泡状棘球蚴在体内的生长
Objective To investigate the role of lymphocytes in the pathogenesis of alveolar hydatid disease in immunoregulation and pathogenesis. Methods BALB / c mice infected by Cysticercus cellulosae were observed for 25 weeks. Spleen cells were prepared at different times and the number of CD + 4 and CD + 8 cells was measured. The changes of CD + 3, CD + 4, CD + 8, CD + 19, CD + 38, CD + 56 and HLA-DR + cells were analyzed by FCM in 25 cases of alveolar echinococcosis and 18 healthy individuals. Results After BALB / c mice were infected with BALB / c mice, the majority of CD + 4 cells were found at 1 to 8 weeks. CD + 4 cells and CD + 8 cells decreased afterwards. The changes of CD8 + cells were significant after 20 weeks / CD + 8 ratio quickly reversed. There was no change in CD + 3 cells in the patients with alveolar echinococcosis. The percentage of CD + 4 / CD8 cells in CD + 4 cells was significantly lower than that in control group (P <0.05) (P <0 05). CD + 56 cells were significantly lower than the normal control group (P <0.01), while CD + 19, CD + 38 and HLA-DR + cells did not change (P> 0.05). Conclusion Cysticercus infection in mice 8 weeks before the CD + 4 cell response, with protective immunity. In late infection gradually to CD + 8 cells, so that the body was immunosuppressed state, is conducive to the survival of metaplasia. Patients with alveolar hydatidosis immunosuppressed state is conducive to the growth of bubble-shaped hydatid cyst in the body