论文部分内容阅读
目的观察人参皂苷C-K对心脏蛋白激酶C信号转导通路的影响,并探讨其作用机制。方法采用oligoGEArray基因芯片技术观察人参皂苷C-K对心脏基因表达的影响;采用放射免疫法观察人参皂苷C-K对体外培养的新生SD大鼠心肌细胞蛋白激酶C活性的影响;采用Straub′s蛙心灌流法观察人参皂苷C-K对蟾蜍离体心脏心肌收缩力的影响。结果人参皂苷C-K可使小鼠心脏基因表达谱发生明显改变,明显上调Prkcα、Prkcε基因表达;可明显增加心肌细胞蛋白激酶C活性,并促进其从细胞浆向细胞膜移位,佛波醇酯和白屈菜赤碱分别能加强和抑制该作用;能够明显增强蟾蜍离体心脏的心肌收缩力,肾上腺素能受体阻断剂拉贝洛尔能抑制该作用。结论人参皂苷C-K可激活心脏蛋白激酶C系统,可能是肾上腺素能受体激动剂。
Objective To observe the effect of Ginsenoside C-K on cardiac protein kinase C signal transduction pathway and to explore its mechanism. Methods The effect of ginsenoside CK on cardiac gene expression was observed by oligoGEArray microarray. The effect of ginsenoside CK on the protein kinase C activity of cardiomyocytes cultured in vitro was observed by radioimmunoassay. Straub’s frog heart perfusion method To observe the effect of ginsenoside CK on contractile force of isolated heart of Toad. Results Ginsenoside CK could significantly change the gene expression profile of heart in mice and significantly up-regulate the expression of Prkcα and Prkcε. It could increase the activity of protein kinase C in cardiomyocytes and promote the translocation from the cytoplasm to the cell membrane. Chelerythrine, respectively, can strengthen and inhibit this effect; Can significantly enhance the cardiac contractility of isolated heart toad, adrenergic receptor blocker labetalol can inhibit this effect. Conclusion Ginsenoside C-K activates the cardiac protein kinase C system and may be an adrenergic receptor agonist.