对于含有大量硅质及多糖如酚、酯、萜等其它次生代谢产物的水稻 ,要从其组织中提取高质量的基因组DNA ,一般比较困难 .作者以水稻叶片为材料 ,分别采取了简易提取法 ,CTAB法 ,QIAGEN公司DneasyPlantMiniKits法 ,ShanghaiSangon公司GenomicDNAIsolationKits法 4种方法提取水稻基因组DNA ;并通过质量分数为 0 .8%琼脂糖凝胶电泳和PCR扩增的方法对所提取的DNA样品进行检测 ,将它们在DNA的产量、质量和耗费等方面进行简要比较 ,并根据分子生物学研究工作的实际特点 ,确定了CTAB沉淀法为最佳方法 . For rice containing lots of siliceous and polysaccharides such as phenol, ester, terpene and other secondary metabolites, it is usually difficult to extract high quality genomic DNA from the tissue of rice.With paddy rice leaves as materials, simple extraction , CTAB method, QIAGEN company DneasyPlantMiniKits method, ShanghaiSangon company GenomicDNAIsolationKits method to extract rice genomic DNA; and by mass fraction of 0.8% agarose gel electrophoresis and PCR amplification of the extracted DNA samples were detected , A brief comparison was made on their yield, quality and cost of DNA. According to the actual characteristics of molecular biology research, the best method of CTAB precipitation was determined.