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从1457例腹泻患儿大便中分离出164株(11.25%)弗劳地枸橼酸盐杆菌复合群的菌株,而对照组中分离率仅4.26%,具统计学差异(P<0.05)。为探讨其致病性,采用不耐热性肠毒素(LT)、耐热性肠毒素(ST)基因探针,菌液直接LT-PCR,家兔肠袢结扎和刚果红结合试验检测细菌的腹泻毒力因子,证实47.1%的菌株产生LT,系腹泻重要毒力因子,不具侵袭性因子。LT阳性菌株见于C.freundii,C.braakii,C.werkmanii。初步认为弗劳地枸橼酸盐杆菌与小儿腹泻密切相关,耐药性检测表明10%的菌株产生超广谱β-内酰胺酶。
A total of 164 isolates (11.25%) from the stool of 1457 children with diarrhea were isolated, and the isolation rate was 4.26% in the control group (P <0.05). In order to investigate its pathogenicity, bacterial heat-labile enterotoxin (LT), heat-resistant enterotoxin (ST) gene probe, bacterial direct LT-PCR, rabbit intestinal ligament ligation and Congo red binding assay Diarrhea virulence factors, confirmed that 47.1% of the strains produce LT, Department of diarrhea, an important virulence factor, non-invasive factors. LT positive strains are found in C. freundii, C.braakii, C.werkmanii. Preliminary thought that Fraxinus citric acid bacteria and diarrhea in children are closely related to drug resistance test showed that 10% of the strains produce extended spectrum β-lactamase.