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目的了解支孢属腐生真菌与致病真菌的基因学特征及二者的关系。方法采用溴苯提取法提取基因组DNA。以随机扩增DNA多态性法对16种支孢26株菌的DNA指纹图谱作了分析。结果①共选用10个随机引物进行扩增,筛选出2个具有稳定、清晰DNA扩增带型的引物,即引物2:5’-AGCGTCCTCC-3’,引物5:5’-CAGCACCCAC-3’。②26株菌DNA带型不完全相同,有一定的种间和种内的遗传变异性,遗传相似性约为37.4%。③同种菌具有较高的遗传相似性。④部分腐生菌与致病菌位于同一树组中。结论随机扩增DNA多态性法研究发现支孢属内各种间有一定的遗传相似性,但腐生菌与致病菌的DNA带型未见明显特征性差异。
Objective To understand the genetic characteristics of the causative fungi and pathogenic fungi of Aspergillus and their relationship. Methods Genomic DNA was extracted by bromobenzene extraction. DNA fingerprinting of 26 strains of 16 kinds of spores were analyzed by random amplified polymorphic DNA. Results ① A total of 10 random primers were selected for amplification. Two primers with stable and clear DNA amplification bands were selected, namely primer 2: 5’-AGCGTCCTCC-3 ’, primer 5: 5’-CAGCACCCAC-3’ . ②26 strain DNA pattern is not exactly the same, there is a certain genetic and intraspecific genetic variability, genetic similarity is about 37.4%. ③ same species of bacteria have a higher genetic similarity. ④ part of saprophytes and pathogens in the same tree group. Conclusion The results of random amplified polymorphic DNA (RFLP) analysis showed that there was a certain genetic similarity among the various species in the genus. There was no obvious characteristic difference between DNA strains of saprophytic bacteria and pathogenic bacteria.