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目的:观察Limk1蛋白在缺氧预处理后不同时间段新生大鼠缺氧缺血和对照组脑组织中的表达差异。方法:采用7日龄SD大鼠54只,随机分为于单纯缺血缺氧组18只,假手术对照组18只,缺氧预处理组18只。各组再分为处理后0 h、1d、7d组,每组各6只。用免疫组化法检测观察Limk1在不同组脑组织各部位的表达差异。结果:Limk1蛋白阳性神经元在各组的吸光度:单纯缺血缺氧组0 h(21.658±3.990)、1 d(30.369±5.156)、7 d(41.546±5.677);缺氧预处理组0 h(30.261±4.266)、1d(43.129±5.785)、7 d(56.309±7.198);假手术组0h(14.113±2.983)、1 d(16,098±3.116)、7d(15.983±3.009)。在单纯缺血缺氧组及缺氧预处理组脑组织的表达比假手术组增多(P<0.01)。在同一时间段上,缺氧预处理组比单纯缺血缺氧组Limk1的表达明显增多(P<0.01)。同样在缺血缺氧后,无论是单纯缺血缺氧组还是缺氧处理组,Limk1的表达随着0 h、1、7 d的推移表达渐增多(P<0.05)。结论:缺氧预处理能够增加新生大鼠缺血缺氧性脑组织中Limk1的表达,其提示缺氧预处理后的新生大鼠缺血缺氧的脑组织可能存在更强的神经重塑作用。
OBJECTIVE: To observe the difference of expression of Limk1 protein between hypoxic-ischemic and control groups in neonatal rats at different time points after hypoxic preconditioning. Methods: Fifty-four SD rats aged 7 days were randomly divided into two groups: 18 for hypoxia-ischemia group, 18 for sham operation group and 18 for hypoxia-preconditioning group. Each group was further divided into 0 h, 1 d and 7 d groups after treatment, 6 in each group. Immunohistochemistry was used to detect the expression of Limk1 in different parts of brain tissue. Results: The absorbency of Limk1 positive neurons in each group were as follows: 0 h (21.658 ± 3.990), 1 d (30.369 ± 5.156), 7 d (41.546 ± 5.677), 0 h (30.261 ± 4.266), 1d (43.129 ± 5.785), 7d (56.309 ± 7.198), sham operation group 0h (14.113 ± 2.983), 1d (16,098 ± 3.116), 7d (15.983 ± 3.009). Compared with sham operation group, the expression of brain tissue increased in hypoxia-deficient group and hypoxic preconditioning group (P <0.01). At the same time, the expression of Limk1 in hypoxic preconditioning group was significantly higher than that in hypoxia-ischemia group (P <0.01). Similarly, after ischemia and hypoxia, the expression of Limk1 gradually increased (P <0.05) at 0 h, 1 and 7 d after ischemia and hypoxia, as well as hypoxia. Conclusion: Hypoxic preconditioning can increase the expression of Limk1 in hypoxic-ischemic brain tissue of neonatal rats, suggesting that hypoxic-ischemic preconditioning may have more neuroplastic effects in hypoxic-ischemic brain tissue of newborn rats .