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目的 研究ATP敏感钾通道 (KATP)亚单位在大鼠组织中的表达。方法 逆转录多聚酶链式反应 (RT PCR)检测通道mRNA的表达。结果 左心室有Kir 6 1,Kir 6 2和SUR 2A的表达 ,大脑皮层中Kir 6 1,Kir 6 2 ,SUR 1,SUR2B均有表达 ,主动脉平滑肌有Kir 6 1,Kir 6 2 ,SUR 2B的表达 ,膀胱平滑肌中有Kir 6 1,Kir 6 2 ,SUR 2B的表达。结论 各组织的KATP组成有所不同 ,Kir 6 1、Kir 6 2和SUR 2B亚单位在多组织中广泛表达。
Objective To study the expression of ATP-sensitive potassium channel (KATP) subunits in rat tissues. Methods Reverse transcriptase polymerase chain reaction (RT-PCR) was used to detect the mRNA expression of channel. Results The expressions of Kir 6 1, Kir 6 2 and SUR 2A in the left ventricle were detected. Kir 6 1, Kir 6 2, SUR 1 and SUR 2 B were expressed in the cerebral cortex. Kir 6 1, Kir 6 2, SUR 2B The expressions of Kir 6 1, Kir 6 2 and SUR 2B in bladder smooth muscle were observed. Conclusions The KATP composition of each tissue is different. Kir 6 1, Kir 6 2 and SUR 2B subunits are widely expressed in multiple tissues.