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柑桔黄龙病是最严重的柑桔病害之一,现已威胁世界柑桔产业。已被证实在中国南部该病害通过嫁接和木虱传播。因此黄龙病的流行病学研究就显得尤为重要。本研究中,基于外膜蛋白(omp)基因的PCR-RFLP的方法被用来研究中国南部各柑桔主产区的亚洲韧皮部杆菌的遗传多样性。研究采用不同的限制性内切酶消化各地区的亚洲韧皮部杆菌的omp基因产生了不同的RFLP指纹图谱,并对各地亚洲韧皮部杆菌的omp基因进行克隆测序然后构建系统发育树。结果显示:中国不同地理区域的亚洲韧皮部杆菌具有一定的遗传多样性,即便在某一特定的地区也有一定的差异。序列分析显示中国地区的亚洲韧皮部杆菌有很高的同源性。这些结果对揭示中国柑桔黄龙病的流行病学及制定科学有效的防治策略具有一定的指导意义。
Citrus Huanglongbing is one of the most serious citrus diseases and is now threatening the world citrus industry. It has been confirmed that the disease is spread by grafting and psylla in southern China. Therefore, the epidemiological study of Huanglong’s disease is particularly important. In this study, the PCR-RFLP method based on the outer membrane protein (omp) gene was used to study the genetic diversity of B. phlei in southern citrus major producing areas in southern China. In this study, different restriction endonucleases were used to digest the omp gene of Phylobacter baumannii in different regions to generate different RFLP fingerprints. The omp gene of Phylobacter baumanca in different regions was cloned and sequenced, then the phylogenetic tree was constructed. The results showed that Phylobacterium asiaticum in different geographic regions of China had certain genetic diversity, even in a certain area. Sequence analysis showed that Phylobacterium Asia in China has high homology. These results have certain guiding significance to reveal the epidemiology of citrus Huanglongbing and to formulate a scientific and effective prevention and treatment strategy.