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目的应用微柱凝胶试剂卡、微柱玻璃珠试剂卡、经典试管法同时检测夫妇ABO血型不合的O型孕妇IgG血型抗体效价,探讨微柱凝胶试剂卡、微柱玻璃珠试剂卡检测孕妇血型抗体效价的界值。方法用微柱凝胶试剂卡、微柱玻璃珠试剂卡、经典试管法平行检测O型血孕妇Ig G血型抗体效价。结果微柱凝胶试剂卡、微柱玻璃珠试剂卡以1∶128为界值与试管法1∶64比较,阳性检出率差异无统计学意义(P>0.05)。结论使用微柱凝胶试剂卡、微柱玻璃珠试剂卡检测O型孕妇IgG血型抗体效价的临床参考值应设为≥1∶128。
OBJECTIVE To simultaneously detect the IgG antibody titer of pregnant women with ABO-incompatible O-type in pregnant women by using micro-column gel reagent card, micro-column glass bead reagent card and classic test tube method, and to explore the detection results of micro-column gel reagent card and micro-column glass bead reagent card Pregnant women blood group antibody titers. Methods The titer of Ig G antibodies of pregnant women with O-type blood was detected in parallel with the micro-column gel reagent card, the micro-column glass bead reagent card and the classic test tube method. Results There was no significant difference in the positive detection rate between the micro-column gel reagent card and the micro-column glass bead reagent card with 1:128 as the cut-off value and the test tube method 1:64 (P> 0.05). Conclusion The clinical reference value of microcolumn gel reagent card and microcolumn glass bead reagent card for detecting the IgG antibody titer of O type pregnant women should be set as ≥1:128.