论文部分内容阅读
本文研究了H615肝癌组织AKP同工酶的活性、电泳行为、热稳定性及对L-苯丙氨酸抑制的敏感性。H615肝癌与正常615鼠肝组织AKP酶活性分别为13.87±5.32单位/克组织及31.30±1.62单位/克组织,二者间有显著性差(P<0.01);在淀粉胶和聚丙烯酰胺凝胶电泳上,H615肝癌AKP与正常615小鼠肝、小肠、胎肝及胎盘AKP有不同的迁移率;此外,H615肝癌AKP比正常肝AKP热稳定性强而对L-苯丙氨酸抑制不敏感。H615肝癌AKP同工酶这些质和量上的变化提示H615肝癌组织具有一种特殊的AKP同工酶,这可能是该肿瘤细胞中一个低水平表达的异位基因的表达产物。
In this paper, the activity of AKP isoenzymes, the electrophoretic behavior, the thermal stability and the sensitivity to L-phenylalanine inhibition in H615 hepatoma tissue were studied. The activity of AKP enzyme in hepatic tissue of H615 and normal 615 mice was 13.87±5.32 units/g and 31.30±1.62 units/g, respectively. There was a significant difference between the two groups (P<0.01); in starch gel and polyacrylamide gels. Electrophoresis, H615 liver cancer AKP and normal 615 mice liver, small intestine, fetal liver and placental AKP have different mobility; In addition, H615 liver cancer AKP heat stability than normal liver AKP but not sensitive to L-phenylalanine inhibition . The qualitative and quantitative changes of AKP isoenzymes in H615 liver cancer suggest that H615 hepatoma tissue has a special AKP isoenzyme, which may be an expression product of a low-level expression of the heterologous gene in the tumor cells.