论文部分内容阅读
目的研究喉癌耐药细胞系(LSC-1/TAX)中MDR1基因表达被沉默后肿瘤细胞对紫杉醇凋亡诱导作用的反应变化。方法表达MDR1 shRNA的慢病毒颗粒转染喉癌耐药细胞系LSC-1/TAX,观察干扰前后紫杉醇诱导喉癌细胞的凋亡改变。采用琼脂糖凝胶电泳进行凋亡定性实验;Giemsa染色观察凋亡细胞的形态学改变;Annexin V-APC/7-AAD双染后,流式细胞仪对凋亡细胞进行定量。结果药敏组细胞发生早期凋亡百分比为(50.95±4.47)%,实验组为(51.04±3.96)%,耐药组为(8.60±1.25)%,对照组为(9.05±1.57)%。实验组与耐药组差异有统计学意义(P<0.05)。喉癌耐药细胞系中MDR1表达被沉默后,细胞对紫杉醇的敏感性提高,凋亡增加。结论表达MDR1 shRNA的慢病毒颗粒可有效地干扰喉癌耐药细胞系中MDR1基因表达,提高喉癌细胞对紫杉醇的凋亡敏感性。
Objective To study the changes of tumor cells response to paclitaxel apoptosis induced by MDR1 gene silencing in the LSC-1 / TAX cell line. METHODS: The lentiviral particles expressing MDR1 shRNA were transfected into LSC-1 / TAX cells, and the apoptosis of laryngeal carcinoma cells was observed before and after paclitaxel intervention. The morphological changes of apoptotic cells were observed by Giemsa staining. The apoptotic cells were quantified by flow cytometry after Annexin V-APC / 7-AAD double staining. Results The percentage of early apoptotic cells was (50.95 ± 4.47)% in experimental group and (51.04 ± 3.96)% in drug sensitive group, (8.60 ± 1.25)% in drug resistant group and (9.05 ± 1.57)% in control group. The difference between the experimental group and the drug-resistant group was statistically significant (P <0.05). MDR1 expression in laryngeal cancer drug-resistant cell lines was silenced, the sensitivity of the cells to paclitaxel increased, apoptosis increased. Conclusion The lentiviral particles expressing MDR1 shRNA can effectively interfere MDR1 gene expression in laryngeal cancer cell lines and increase the sensitivity of paclitaxel to laryngocarcinoma cells.