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目的:探讨清毒片联合端粒酶逆转录酶反义核酸(hTERT ASODN)对HL-60细胞增殖及凋亡的影响。方法:体外培养HL-60细胞,分别加入清毒片、hTERT ASODN、清毒片加hTERT ASODN,取培养24、48、72h的细胞,采用四唑盐比色(MTT)法检测细胞增殖、Annexin V-FITC/PI双染流式细胞术检测细胞凋亡。以加入阿糖胞苷组为阳性对照,未处理的细胞为空白对照。结果:清毒片、hTERT ASODN(0.5μmol/L)、阿糖胞苷(10μmol/L)均能抑制HL-60细胞增殖、诱导细胞凋亡,其作用随着培养时间的延长增强;HL-60细胞增殖抑制率及诱导凋亡率从高到低依次为:阿糖胞苷组>清毒片加hTERT ASODN组>hTERT ASODN组>清毒片组;清毒片加hTERT ASODN对HL-60细胞的增殖抑制及诱导凋亡作用明显高于单独使用清毒片或hTERT ASODN(P<0.05)。结论:清毒片与hTERT ASODN联合对于抑制HL-60细胞增殖及诱导凋亡具有协同效应。
Objective: To investigate the effect of hTERT ASODN on the proliferation and apoptosis of HL-60 cells. Methods: HL-60 cells were cultured in vitro, and hTERT ASODN, hTERT ASODN and hTERT ASODN were added respectively. The cells were cultured for 24, 48 and 72 hours. The cell proliferation was detected by tetrazolium salt (MTT) V-FITC / PI double staining flow cytometry was used to detect apoptosis. To add cytarabine group as a positive control, untreated cells as a blank control. Results: The cells of HL-60, hTERT ASODN (0.5μmol / L) and cytarabine (10μmol / L) all inhibited the proliferation and induced the apoptosis of HL-60 cells. 60 cell proliferation inhibition rate and induction of apoptosis in descending order were: cytarabine> detoxification plus hTERT ASODN group> hTERT ASODN group> Qingdu Tablet group; Qingdu Tablet plus hTERT ASODN HL-60 Cell proliferation inhibition and induction of apoptosis were significantly higher than those treated with Qingdu tablets alone or hTERT ASODN (P <0.05). Conclusion: The combination of Qingdu Tablet and hTERT ASODN has a synergistic effect on inhibiting the proliferation and inducing apoptosis of HL-60 cells.