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供试材料计有本地及国外引进大麦品种十九种。以成熟种子之胚为培殖体,采用MS基本培养基,在愈伤组织诱导中,加入不同量的有机成份及不同的植物激素包括0.1～2.0mg/L的2.4—D或0.25mg/L的2,4,5—T或0.5～1.0mg/L的Kinetin。继代培养则加2mg/L的IAA及1.0～10mg/L的2.4—D。再生培养基为(1/2)MS或MS内加1mg/L的BA与2mg/L的Adenine。结果显示,在19个品种中,有15种产生愈伤组织。愈伤组织的形成似以培养基内加2mg/L的2.4—D与1mg/L的Kinetin效果最佳,但仍依基因型不同而有差异。其中以中兴二号及Trompillo效果最佳。所产生的
The test materials include 19 varieties of barley introduced locally and abroad. The embryos of mature seeds were used as the culture medium. MS basal medium was used. In callus induction, different amount of organic components and different phytohormones including 0.1 ~ 2.0mg / L 2.4-D or 0.25mg / L Of 2,4,5-T or 0.5-1.0 mg / L of Kinetin. Subculture with 2mg / L of IAA and 1.0 ~ 10mg / L of 2.4-D. The regeneration medium was (1/2) MS or MS plus 1 mg / L BA and 2 mg / L Adenine. The results showed that of the 19 cultivars, 15 produced callus. The formation of callus seems to be the best medium with 2mg / L of 2.4-D and 1mg / L of Kinetin, but still vary by genotype. Among them ZTE II and Trompillo works best. produced