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目的:探讨间断性低氧暴露及复氧休息对红细胞参数及血清低氧诱导因子-1α(HIF-1α)、促红细胞生成素(EPO)的影响。方法:制备间断性低氧动物模型,检测全血RBC、Hb、HCT红细胞参数,采用ELISA法检测血清HIF-1α、EPO水平,结合现场调查,检测间断性高原作业人员红细胞参数及EPO水平。结果:IH7、14、21、28d组大鼠RBC、Hb、HCT水平明显高于常氧对照组(P<0.05),复氧后各参数水平下降。IH3、7、14d组HIF-1α高于常氧对照组(P<0.05),EPO在IH3、7d组高于对照组(P<0.05),复氧后HIF-1α、EPO水平下降。8个月组高原作业人员RBC水平高于平原对照组(P<0.05),Hb在2年组高于平原对照组(P<0.05)。HCT与RBC大致呈同一规律,且2年组HCT仍明显高于平原对照组(P<0.05)。与平原对照组比较,各组EPO的差异不显著。结论:间断性低氧暴露可以增加血清HIF-1α、EPO的含量,提高红细胞数量和血红蛋白的浓度,并随低氧周期的延长存在一定变化规律;复氧休息有利于低氧后机体的调整,使升高的红细胞参数及HIF-1、EPO水平下降。
Objective: To investigate the effects of intermittent hypoxic exposure and reoxygenation on erythrocyte parameters, serum hypoxia inducible factor-1α (HIF-1α) and erythropoietin (EPO). Methods: The animal model of intermittent hypoxia was established. The parameters of whole blood RBC, Hb and HCT were detected. The serum levels of HIF-1α and EPO were detected by ELISA. The erythrocyte parameters and EPO levels of intermittent plateau were detected by field investigation. Results: The levels of RBC, Hb and HCT in IH7, 14, 21, 28 d group were significantly higher than those in normoxic control group (P <0.05). The levels of HIF-1α in IH3,7,14d group were higher than those in normoxia control group (P <0.05). The levels of EPO in IH3,7d group were higher than those in control group (P <0.05). The level of RBC in 8-month group was higher than that in plain control group (P <0.05), and Hb was higher in 2-year group than in plain control group (P <0.05). HCT and RBC roughly the same law, and 2-year HCT was still significantly higher than the plain control group (P <0.05). Compared with plain control group, the difference of EPO in each group was not significant. Conclusion: Intermittent hypoxic exposure can increase the levels of serum HIF-1α and EPO, increase the number of erythrocytes and hemoglobin, and change with the prolongation of hypoxia cycle. Resting oxygen is beneficial to the body’s regulation after hypoxia, Reduce elevated erythrocyte parameters and HIF-1, EPO levels.