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以东乡野生稻/协青早B∥协青早B的回交自交系群体为遗传材料,利用简化基因组测序技术鉴定群体的SNP分子标记基因型,对东乡野生稻的粒形相关性状进行QTL分析。在水稻第1,3,4,5,10,12号染色体上共发现14个粒形相关QTL,包括6个粒长QTL(q GL1、q GL3.1、q GL3.2、q GL4、q GL10.1、q GL10.2)、2个粒厚QTL(q GT5、q GT10)和6个粒重QTL(q KGW3、q KGM4、q KGW5、q KGW 10.1、q KGW10.2、q KGW12)。其中q GL3.1与q KGW3、q GT5与q KGW5、q GL4与q KGM4、q GL10.1与q KGW10.1以及q GL10.2、q GT10与q KGW10.2定位区间重叠,推测这些QTL具有一因多效特性。加性效应最大的粒长QTL即q GL3.1(q KGW3)被定位在第3染色体的粒形基因GS3的区域中,且q GL3.1与GS3都是控制粒长的主效QTL,推测q GL3.1就是GS3基因。而在q GL3.1区域附近还存在粒长QTL q GL3.2,该位点与已克隆的粒长QTL即GL3.1在位置上重叠,推测q GL3.2可能就是GL3.1/Os PPKL1基因。
The backcrossing inbred lines of Dongxiang wild rice / Xieqingzao B∥ Xieqingzao B were used as genetic material, and the SNP molecular marker genotypes were identified by using simplified genome sequencing. QTLs for grain-shape-related traits in Dongxiang wild rice analysis. Fourteen QTLs related to grain shape were found on chromosomes 1, 3, 4, 5, 10 and 12, including 6 grain length QTLs (q GL1, q GL3.1, q GL3.2, q GL4, q (Q GT5, q GT10) and 6 grain weight QTL (q KGW3, q KGM4, q KGW5, q KGW 10.1, q KGW10.2, q KGW12) . Among them, q GL3.1 and q KGW3, q GT5 and q KGW5, q GL4 and q KGM4, q GL10.1 and q KGW10.1 and q GL10.2 and q GT10 and q KGW10.2 overlapped with each other, suggesting that these QTLs Has a multi-effect characteristics. The most additive QTL, q GL3.1 (q KGW3), is located in the region of the GS3 gene on chromosome 3, and both q GL3.1 and GS3 are major QTLs controlling grain length. q GL3.1 is the GS3 gene. In the vicinity of q GL3.1, there is also QTL qTL q3, which is overlapped with the cloned grain length QTL, namely GL3.1. It is speculated that q GL3.2 may be GL3.1 / Os PPKL1 gene.