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猕猴桃茎段产生的愈伤组织，经过冷驯化处理后，加入５％ＤＭＳＯ和１０％葡萄糖，以一１℃·ｍｉｎ~(-１)降温速率降至一６０一－７５℃，投入液氮中保存。在液氮中存放时间的长短对愈伤组织存活率没有影响，在３５一４０℃水浴中迅速化冻，将化冻后的材料接种在ＭＳ附加０．０５ｍｇ·１~(-１)２，４一Ｄ及１．０ｍｇ·１~(-１)ＺＴ的培养基上暗培养，待开始恢复生长转在光下培养１５一２０ｄ，再转接在只含有１．０ｍｇ·１~(-１)ＺＴ的ＭＳ分化培养基上，２０一３０ｄ后分化出芽，并形成植株。
After the callus was cold acclimated, 5% DMSO and 10% glucose were added into the callus of the kiwifruit stem, and the callus was cooled down to a temperature of 60-75 ° C at a rate of 1 ℃ · min -1 (-1) save. The storage time in liquid nitrogen had no effect on the survival rate of callus. The frozen thawed material was rapidly thawed in a water bath at 35-40 ℃ and inoculated into MS with 0.05mg · 1 -1 2,4 D and 1.0mg · 1 ~ (-1) ZT on dark medium, and then resume growth at 15 ~ 20d after light and then transferred to culture medium containing only 1.0mg · 1 ~ (-1) ZT On MS differentiation medium, buds differentiated after 20-30 days and formed plants.