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目的:研究不同纯化技术对甘草水提液的纯化效果,为中药水提液的纯化提供参考。方法:分别采用微滤与超滤,Ⅱ型ZTC 1+1天然澄清剂、壳聚糖、微滤分别与DA-201型大孔吸附树脂联用共4种联用技术对甘草水提液进行分离纯化,以指标性成分(甘草苷和甘草酸)的保留率、物理化学参数及HPLC指纹图谱等指标综合分析以上方法的适用性。结果:联用技术会使指标性成分的含量降低,但可有效去除了大分子物质。在单用纯化技术中,微滤技术纯化效果最好;微滤与超滤联用得到的纯化液与原液的相似度0.886,在4种联用纯化技术中对药液组分影响最小。结论:微滤与超滤联用技术或许是较好的中药水提液分离纯化方法,该方法避免了有机试剂对中药水提液的污染,且操作简便、节省成本,适合工业化连续生产。
OBJECTIVE: To study the purification effect of different purification techniques on water extract of Glycyrrhiza uralensis, and to provide reference for the purification of traditional Chinese medicine aqueous extract. Methods: Four kinds of combined technologies of microfiltration and ultrafiltration, type Ⅱ ZTC 1 + 1 natural clarifier, chitosan, microfiltration and macroporous adsorption resin DA-201, respectively Separation and purification, the index of the ingredients (liquiritin and glycyrrhizic acid) retention rate, physical and chemical parameters and HPLC fingerprints and other indicators comprehensive analysis of the applicability of the above method. Results: Combined technology can reduce the content of the index components, but can effectively remove the macromolecules. In the single purification technology, microfiltration technology has the best purification effect. The similarity between the purified solution and the original solution obtained by the combination of microfiltration and ultrafiltration is 0.886, which has the least influence on the liquid components in the four kinds of combined purification technologies. Conclusion: The combination of microfiltration and ultrafiltration may be a better method for the separation and purification of traditional Chinese medicine aqueous extracts. This method avoids the contamination of traditional Chinese medicine aqueous extracts by organic reagents and is simple, cost-effective and suitable for industrial continuous production.