论文部分内容阅读
目的探讨不同毒力的弓形由株与其致病性的关系、方法应用体外培养和动物模型观察ＲＨ、Ｂ３６、Ｆｕｋａｙａ三虫株对组织细胞的损害及其致病、致畸、致死的差异。结果Ｖｅｒｏ细胞在８ｈ内的侵袭率及分解产物脂肪酸含量以Ｂ３６株为高。胞内２４ｈ繁殖数以ＲＨ株为最多，Ｂ３６次之，Ｆｕｋａｙａ最低。此结果与虫体对细胞的损害程度、对仔鼠智力发育的影响密切相关。细胞因子激活巨噬细胞后，对弓形由抑虫或杀虫程度与ＮＯ浓度相一致。结论ＲＨ株ＮＯ浓度高，胞内虫株可被完全消灭，ＮＯ可能参与抗虫作用。
Objective To investigate the relationship between pathogenicity and arcuate strains of different virulence. Methods The in vitro culture and animal models were used to observe the damage to the cells and the pathogenicity, teratogenicity and lethality of RH, B36 and Fukaya three strains. Results The invasion rate of Vero cells within 8h and the fatty acid content of decomposed products were high in B36 strain. 24h intracellular multiplication RH strain is the most, B36 second, Fukaya lowest. This result is closely related to the degree of damage to cells by parasites and the effect on the mental development of offspring. After macrophages are activated by cytokines, the degree of inhibition or insecticidality of the arches is consistent with the concentration of NO. Conclusion The RH strain has high concentration of NO, the intracellular strains can be completely eliminated, NO may be involved in the anti-insect effect.