论文部分内容阅读
本研究观察了雌二醇和Ａｎｏｒｄｉｏｌ（一种有激动剂活性的抗雌激素药物）对未成年雌性大鼠子宫水通道（ＡＱＰＣＨＩＰ）基因表达的调控作用。我们根据两种大鼠ＡＱＰＣＨＩＰ水通道ｃＤＮＡ保守序列设计合成了一对寡核苷酸引物，用于扩增从大鼠子宫总ＲＮＡ反转录而成的ｃＤＮＡ片段。给未成年大鼠用单剂量的雌二醇（４０μｇ·ｋｇ－１）９ｈ后，ＡＱＰＣＨＩＰｍＲＮＡ的表达量显著增加。雌二醇和Ａｎｏｒｄｉｏｌ的最低有效量分别为４０μｇ·ｋｇ－１和５０μｇ·ｋｇ－１，但Ａｎｏｒｄｉｏｌ的刺激作用比雌二醇强。本文结果提示，ＡＱＰＣＨＩＰ水通道基因的表达可能与雌二醇和Ａｎｏｒｄｉｏｌ介导的子宫水的浸渗作用以及子宫腔内液体的产生有关
This study examined the regulation of the expression of the AQP-CHIP gene in female adult rats using estradiol and Anordiol, an agonist-active antiestrogen. We designed and synthesized a pair of oligonucleotide primers based on the conserved sequences of two rat AQP-CHIP aquaporins to amplify cDNA fragments reverse transcribed from rat uterine total RNA. The expression of AQPCHIP mRNA was significantly increased after a single dose of estradiol (40 μg · kg -1) was given to the unaged rats for 9 h. The minimum effective doses of estradiol and Anordiol were 40 μg · kg-1 and 50 μg · kg-1, respectively, but the irritation of Anordiol was stronger than that of estradiol. The results suggest that the expression of AQP CHIP channel may be related to estradiol and Anordiol-mediated uterine water infiltration and uterine cavity fluid production