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目的:为了探讨断奶仔小鼠应激腹泻发生中5-HT和VIP之间的互作机制。方法:将21 d断奶ICR小鼠分为正常对照组,应激腹泻组(番泻叶0.4 kg/L按体重15mL/kg灌胃同时后肢束缚应激),CH药物对照组(氢溴酸西酞普兰,是一种高选择性5-HT再摄取抑制剂,抑制5-HT再摄取灭活过程,使5-HT含量增加。10 mg/kg体重溶解于0.1 mL生理盐水,腹腔注射),CH+应激腹泻组(CH药物处理4h后进行应激腹泻处理),PCPA对照组(对氯苯丙氨酸,是一种5-HT合成抑制剂,抑制5-HT合成,300 mg/kg溶解于0.1 mL生理盐水,腹腔注射),PCPA+应激腹泻组。常规饲养。处理5d后小鼠脱颈椎死,取十二指肠、空肠、回肠、结肠组织保存于-80℃用作放射免疫检测。放射免疫测定十二指肠,空肠,回肠和结肠中VIP的表达量。结果:十二指肠中,与对照组相比,应激腹泻组小鼠VIP的表达量升高了38.78%,差异显著(P=0.000);PCPA组小鼠VIP的表达量降低了16.91%,差异显著(P=0.034);CH组小鼠VIP的表达量升高了65.60%,差异显著(P=0.000)。与应激腹泻组相比,PCPA+应激腹泻组小鼠VIP的表达量升高了8.61%,差异不显著(P=0.122);CH+应激腹泻组VIP的表达量升高到(5.99±0.08)pg/mgpro,差异显著(P=0.000)。空肠中,与对照组(0.51±0.02)pg/mgpro相比,应激腹泻组小鼠VIP的表达量升高了15.68%,差异不显著(P=0.308);PCPA组VIP的表达量降低到了(0.39±0.02)pg/mgpro,差异不显著(P=0.155);CH组小鼠VIP的表达量升高了25.49%,差异不显著(P=0.109)。与应激腹泻组(0.59±0.11)pg/mgpro相比,PCPA+应激腹泻组小鼠VIP的表达量降低,但差异不显著(P=0.428);CH+应激腹泻组VIP的表达量升高了40.68%,差异显著(P=0.009)。回肠中,与对照组(0.50±0.06)pg/mgpro相比,应激腹泻组小鼠VIP的表达量升高,差异不显著(P=0.582);PCPA组小鼠VIP的表达量降低了22.00%,差异显著(P=0.030);CH组VIP的表达量升高了32.00%,差异显著(P=0.003)。与应激腹泻组相比,PCPA+应激腹泻组表达量升高了25%,差异显著(P=0.012);CH+应激腹泻组VIP的表达量升高了42.31%,差异显著(P=0.001)。结肠中,与对照组相比,应激腹泻组小鼠VIP的表达量升高了5.88%,差异不显著(P=0.607);PCPA组表达量降低了26.47%,差异显著(P=0.044);CH组的表达量无变化。与应激腹泻组相比,PCPA+应激腹泻组小鼠VIP的表达量升高了37.50%,差异显著(P=0.005);CH+应激腹泻组小鼠VIP的表达量升高了6.94%,差异不显著(P=0.572)。结论:总之,断奶仔小鼠应激腹泻导致肠道VIP的表达量升高;PCPA诱导5-HT降低后,VIP的表达量出现显著降低,而CH诱导5-HT升高后VIP的表达量亦出现显著升高。因此,5-HT和VIP在断奶仔小鼠应激腹泻发生中呈现高度相关性。
Objective: To investigate the mechanism of interaction between 5-HT and VIP during stress diarrhea in weanling mice. Methods: The 21 d wean ICR mice were divided into normal control group, stress diarrhea group (0.4 mL / L of senna by body weight 15 mL / kg intragastric administration of hind limb restraint stress), CH drug control group Phlopram, a highly selective inhibitor of 5-HT reuptake, inhibits 5-HT reuptake inactivation and increases 5-HT content. 10 mg / kg body weight is dissolved in 0.1 mL saline and injected intraperitoneally) CH + stress diarrhea group (stress diarrhea treated 4 h after CH drug treatment), PCPA control group (p-chlorophenylalanine, a 5-HT synthesis inhibitor, inhibition of 5-HT synthesis, 300 mg / kg dissolution In 0.1 mL saline, intraperitoneal injection), PCPA + stress diarrhea group. Routine feeding. The mice were sacrificed 5 days after the treatment of cervical spondylosis, take the duodenum, jejunum, ileum, colon tissue stored at -80 ℃ for radioimmunoassay. Radioimmunoassay for the expression of VIP in the duodenum, jejunum, ileum and colon. Results: Compared with the control group, the expression of VIP in the diarrhea group increased by 38.78% (P = 0.000); the expression of VIP in the PCPA group decreased by 16.91% (P = 0.034). The expression of VIP in CH group increased by 65.60% (P = 0.000). Compared with the stress diarrhea group, the expression of VIP in PCPA + diarrhea group increased by 8.61% (P = 0.122); the expression of VIP in CH + stress diarrhea group increased to (5.99 ± 0.08) ) pg / mgpro, significant difference (P = 0.000). Compared with the control group (0.51 ± 0.02) pg / mgpro, the expression of VIP in the diarrhea group was increased by 15.68% (P = 0.308). The expression of VIP in the PCPA group was reduced to (0.39 ± 0.02) pg / mgpro, the difference was not significant (P = 0.155). The expression of VIP in CH group increased by 25.49%, but the difference was not significant (P = 0.109). Compared with the stress diarrhea group (0.59 ± 0.11) pg / mgpro, the expression of VIP in PCPA + stress diarrhea group decreased, but the difference was not significant (P = 0.428); The expression of VIP in CH + stress diarrhea group increased 40.68%, significant difference (P = 0.009). Compared with the control group (0.50 ± 0.06) pg / mgpro, the expression of VIP in stress diarrhea group was not significantly different (P = 0.582); the expression of VIP in PCPA group decreased by 22.00 %, The difference was significant (P = 0.030). The expression of VIP in CH group increased by 32.00%, the difference was significant (P = 0.003). Compared with stress diarrhea group, the expression of VIP in diarrhea group increased by 25% (P = 0.012), and the expression of VIP in diarrhea group increased by 42.31% (P = 0.001) ). Compared with control group, the expression of VIP in colon increased significantly by 5.88% (P = 0.607), while the expression of PCPA decreased by 26.47% (P = 0.044) There was no change in CH group expression. Compared with stress diarrhea group, the expression of VIP in PCPA + stress diarrhea group increased by 37.50% (P = 0.005), the expression of VIP increased by 6.94% in CH + stress diarrhea group, The difference was not significant (P = 0.572). Conclusions: In conclusion, the expression of VIP in the diarrhea-induced stress diarrhea in mice was increased, while the expression of VIP was significantly decreased after PCPA induced the decrease of 5-HT. However, the expression of VIP in CH-induced 5-HT increased Also showed a significant increase. Therefore, 5-HT and VIP are highly correlated in the occurrence of stress diarrhea in weanling mice.