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利用荧光技术对芥菜型油菜(Brassicajuncea)与白菜(B.pekinesis)种间正反杂交后花粉萌发和花粉管生长过程进行了观察。结果显示:芥菜型油菜与白菜正交授粉后,花粉在柱头上能正常萌发,多数花粉管沿花柱到达胚珠完成受精,且受精方式有珠孔受精、合点受精和中部受精,少量花粉管生长不正常,出现花粉管顶端膨大扭曲,花粉管分支等异常现象;反交授粉后,花粉在柱头上萌发时柱头乳突细胞产生强烈胼胝质反应,影响花粉管生长,只有少量花粉管通过花柱到达胚珠完成受精。用石蜡切片技术观察了正反杂交后杂种的胚胎发育,正交杂种胚胎发育较早,胚和胚乳生长较正常,杂种胚一般均能发育至成熟;反交杂种胚发育至心型期便不能继续发育,胚乳也停滞在游离核阶段并最终败育。综合分析表明,芥菜型油菜与白菜正反杂交都存在一定程度的受精不亲和性。
Fluorescence was used to observe the pollen germination and pollen tube growth after positive and negative interspecific hybridization between Brassica juncea and B.pekinesis. The results showed that the pollen germinated on the stigma after orthogonal pollination of Brassica juncea and cabbage. Most of the pollen tube reached the ovule along the style to complete the fertilization, and the fertilization method was the peritoneal fertilization, co-fertilization and central fertilization, and the few pollen tube grew abnormally , There appeared the twisting of the top of the pollen tube and abnormal branching of the pollen tube. After anther cross pollination, the stigma mast cells produced intense callose reaction when the pollen germinated on the stigma and affected the pollen tube growth. Only a few pollen tubes reached the ovule through the style column Fertilization. Paraffin sections were used to observe the hybridization embryos after positive and negative hybridization embryos, hybrid embryos developed earlier, embryo and endosperm growth than normal, hybrid embryos can generally develop to mature; crossbred embryo development to the cardiac phase can not Continue to develop, endosperm also stagnated in the free nuclear phase and eventually aborted. Comprehensive analysis showed that there was some degree of insemination incompatibility between the positive and negative hybrids of Brassica juncea and Brassica campestris.