论文部分内容阅读
用皮下注射吗啡 ,建立雄性大鼠吗啡依赖模型。戒断组给吗啡依赖大鼠侧脑室注射乌拉地尔( IVC)。阳性对照组给正常大鼠 IVC,阴性对照组给正常大鼠 IVC相同体积生理盐水。用免疫组织化学方法观察吗啡依赖大鼠 ,IVC戒断大鼠和 IVC正常大鼠垂体远侧部 TSH细胞的变化。结果发现 ,吗啡依赖大鼠和IVC戒断大鼠 TSH免疫反应细胞的面数密度减少 ,免疫反应减弱 ( P <0 .0 1)。阳性对照组大鼠 TSH免疫反应细胞的面数密度增大 ,免疫反应增强 ( P <0 .0 5 )。本文对吗啡及乌拉地尔所引起大鼠垂体远侧部 TSH免疫反应细胞变化机理进行了探讨
Morphine dependence was established by subcutaneous injection of morphine in male rats. Intracerebroventricular injection of urapidil (IVC) into morphine-dependent rats was performed in the withdrawal group. Positive control group to normal rats IVC, negative control group to normal rats IVC the same volume of normal saline. The changes of TSH cells in the distal pituitary of morphine dependent rats, IVC withdrawal rats and IVC normal rats were observed by immunohistochemistry. The results showed that the number density of TSH immunoreactive cells in morphine-dependent rats and IVC withdrawal rats decreased, and the immune response decreased (P <0.01). The number density of TSH immunoreactive cells in the positive control group increased, and the immune response increased (P <0.05). In this paper, morphine and urapidil induced rat distal pituitary TSH immune response to changes in the mechanism were discussed