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利用单克隆抗体ＡＥ５与分化型角膜上皮细胞中角蛋白Ｋ３特异性结合，研究缺乏分化标志特征的角膜上皮干细胞定位特点，应用免疫组织化学方法显示Ｋ３阳性表达的区域分布于除角膜缘上皮基底部以外的所有角膜上皮细胞中，角膜上皮干细胞存在于角膜缘基底部ＡＥ５抗体反应阴性细胞中，即角膜干细胞位于角膜缘上皮层基底部。
Using the monoclonal antibody AE5 and keratin K3 in differentiated corneal epithelial cells specifically binding to study the characteristics of the lack of differentiation markers of corneal epithelial stem cell localization characteristics of the use of immunohistochemical staining K3 positive expression of the region in addition to the limbal epithelial base In all corneal epithelial cells except corneal epithelial cells, corneal epithelial stem cells are present in the corneal bottom of AE5 antibody negative cells, that corneal stem cells located in the corneal limbal basement.