论文部分内容阅读
目的分析武汉市硚口区水产品中霍乱弧菌的污染状况,为霍乱防治工作提供实验室依据。方法依据第6版《霍乱防治手册》和霍乱诊断标准WS289-2008中的有关要求进行霍乱弧菌的分离培养、生化鉴定、血清学检测、分子生物学检测。结果 2004-2013年共检测出15株霍乱弧菌,其中稻叶型霍乱弧菌毒力株5株、稻叶型霍乱弧菌无毒力株8株、检测出2株与O1、O139群血清有交叉凝集现象的O71群霍乱弧菌。结论近年来检测出的稻叶型霍乱弧菌虽然大部分无毒力基因,但仍应加强对水产品的主动监测,以明确霍乱防控工作重点,制定防控策略。基层实验室在遇到霍乱弧菌血清凝集实验阳性的可疑菌时,应及时送上级疾控部门做进一步的生化和分子生物学检测,以得到准确的结果。此外,水产品霍乱弧菌检出率与采样质量密切相关,标本采集人员应接受专门的采样培训。
Objective To analyze the status of Vibrio cholerae contamination in aquatic products in Xiangkou District of Wuhan City and provide a laboratory basis for the prevention and control of cholera. Methods Vibrio cholerae isolation and culture, biochemical identification, serological test and molecular biological test were conducted according to the 6th Edition Cholera Prevention and Control Manual and the cholera diagnostic standard WS289-2008. Results A total of 15 strains of V. cholera were detected in 2004-2013, including 5 strains of V. cholerae virulent strains and 8 strains of non-virulent strains of V. cholerae. Two strains were detected, O71 group Vibrio cholerae with cross-aggregation. Conclusion Although most of the avirulent strains of V. cholerae detected in recent years have no virulence genes, active monitoring of aquatic products should be strengthened in order to clarify the focus of prevention and control of cholera and formulate prevention and control strategies. Primary laboratory in the encounter Vibrio cholerae serum agglutination test positive suspicious bacteria should be promptly sent to higher level disease control department for further biochemical and molecular biology tests to get accurate results. In addition, the detection rate of Vibrio cholerae in aquatic products is closely related to the quality of sampling, and specimen collection personnel should receive specialized sampling training.