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目的探讨金雀异黄素(genistein,Gen)诱导人白血病Jurkat E6-1细胞凋亡机制。方法以不同浓度Gen作用于Jurkat E6-1细胞,采用四甲基偶氮噻唑蓝(MTT)法检测Gen对Jurkat E6-1细胞增殖抑制作用;采用DNA-ladder检测Gen对细胞凋亡影响;采用实时定量PCR(RT-PCR)检测凋亡相关基因bax、bcl-2表达水平。结果与对照组比较,P>0.5μmol/L浓度的Gen明显抑制Jurkat E6-1细胞增殖,培养24 h,10μmol/L Gen组抑制率为5.9%,与对照组比较,差异有统计学意义(P<0.01),随Gen浓度增加和培养时间延长,抑制作用逐渐增强,72 h后,10μmol/L Gen组抑制率为24.9%;Gen使Jurkat E6-1细胞Bax表达上调,Bcl-2表达下调,且随着浓度增加,作用增强。结论 Gen对人白血病Jurkat E6-1生长具有明显抑制作用,可诱导Jurkat E6-1细胞凋亡。
Objective To investigate the mechanism of genistein (Gen) inducing apoptosis in human leukemia Jurkat E6-1 cells. Methods Jurkat E6-1 cells were treated with different concentrations of Gen and the proliferation of Jurkat E6-1 cells was detected by MTT assay. The effects of Gen on apoptosis were detected by DNA-ladder. Real-time quantitative PCR (RT-PCR) was used to detect the expression of bax and bcl-2. Results Compared with the control group, Gen (P> 0.5μmol / L) significantly inhibited the proliferation of Jurkat E6-1 cells. After cultured for 24 h, the inhibitory rate of 5.9% in 10μmol / L Gen group was significantly higher than that of the control group P <0.01). With the increase of Gen concentration and culture time, the inhibition increased gradually. After 72 h, the inhibition rate of 10μmol / L Gen group was 24.9%. Gen up-regulated Bax expression and down-regulated Bcl-2 expression in Jurkat E6-1 cells , And with increasing concentration, the effect is enhanced. Conclusions Gen on human leukemia Jurkat E6-1 significantly inhibited the growth and induced Jurkat E6-1 cell apoptosis.