用免疫微球技术来检测抗原抗体是目前免疫诊断试剂研究的一个新方向．本文介绍了兔抗人血清ＩｇＧ的制备和利用彩色微球检测该抗体的检测方法；同时，探讨了影响实验敏感性的温度、浓度和ｐＨ值等主要因素． Immunoglobulin technology to detect antigen antibodies is a new direction of immunodiagnostic reagents. This article describes the preparation of rabbit anti-human serum IgG and the detection method of the antibody using color microspheres. At the same time, the main factors affecting the sensitivity of the experiment such as temperature, concentration and pH are discussed.