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背景与目的:肺癌染色体异常与临床反应及临床预后密切相关。通过对肺癌手术后高生存期患者外周血淋巴细胞和肺癌组织进行染色体分析,为肺癌发生发展相关基因的筛选及肺癌临床治疗奠定基础。方法:选择肺癌高生存期组术后生存5年以上的肺癌患者20例,其中鳞状细胞癌9例、腺癌6例、小细胞癌5例。肺癌对照组随机选择术后1年内患者20例,其中鳞状细胞癌8例、腺癌7例、小细胞癌5例。正常对照组选择健康志愿者20例。常规法进行以上3组患者的外周血淋巴细胞中期染色体分析。肺癌高生存组和肺癌对照组选择石蜡组织包埋块提取基因组DNA,DeVries法进行CGH分析。结果:高生存期组外周血淋巴细胞染色体畸变发生率为3.15%,显著低于肺癌对照组的10.85%(P<0.01),染色体畸变主要为染色体单体裂隙、断片、无着丝粒片段,偶见衍生染色体。CGH分析结果显示,高生存期组有6例未见任何染色体畸变发生,肺癌对照组有1例未见染色体畸变发生。高生存期组畸变染色体平均数目(2.60±1.85)显著少于肺癌对照组(5.10±2.13)。结论:染色体畸变少的肺癌患者预后好、生存期长,肺癌临床高生存期可能与染色体遗传稳定性相关。
BACKGROUND & OBJECTIVE: Chromosomal abnormalities in lung cancer are closely related to clinical response and clinical prognosis. Chromosomal analysis of peripheral blood lymphocytes and lung cancer tissues in patients with high survival after lung cancer surgery lays the foundation for the screening of genes involved in the development of lung cancer and the clinical treatment of lung cancer. Methods: Twenty lung cancer patients who survived more than 5 years after surgery were selected, including 9 cases of squamous cell carcinoma, 6 cases of adenocarcinoma and 5 cases of small cell carcinoma. The control group of lung cancer were randomly selected 20 patients within 1 year after operation, including squamous cell carcinoma in 8 cases, adenocarcinoma in 7 cases and small cell carcinoma in 5 cases. Normal control group of 20 healthy volunteers. Conventional method for the above three groups of peripheral blood lymphocytes metaphase analysis. High lung cancer survival group and lung cancer control group were selected paraffin embedded block genomic DNA extraction, DeVries method CGH analysis. Results: The incidence of chromosome aberrations in peripheral blood lymphocytes in high survival group was 3.15%, which was significantly lower than that in control group (10.85%, P <0.01). Chromosome aberrations were mainly chromosome cleft, fragment, Occasionally derived chromosomes. CGH analysis showed that 6 cases of high survival group did not occur any chromosomal aberrations, lung cancer control group, 1 case no chromosomal aberrations. The average number of aberrant chromosomes in the high survival group (2.60 ± 1.85) was significantly less than that in the lung cancer control group (5.10 ± 2.13). Conclusion: The prognosis of long-term survival and long-term survival of lung cancer patients with few chromosomal aberrations may be related to the genetic stability of chromosomes.