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提高微量检材的利用率 ,建立PEP方法并对其法医学应用进行可行性研究。用 15个随机寡核苷酸的序列作为引物 ,低特异性下扩增出微量DNA ,以此扩增物作为模扳 ,进行特异基因座扩增。经PMCT118,GABAR ,DYS19,D18S849,D3S1744,D12S10 90 ,D8S1179,D2 1S11,D18S5 1,D5S818,D13S3 17,D7S82 0 ,D3S13 5 8,vWA ,FGA ,TH0 1,CSF1PO ,TPOX ,D16S5 3 9及Amelogene等 2 0个基因座的PEP PCR与直接的PCR分析比较 ,二者分型结果一致 ,即使 1ngDNA也能得到正确分型。PEP方法可用于法医学检验 ,有利于微量物证的利用 ,使物证检材提供尽可能多的信息
Improve the utilization rate of trace materials, establish PEP method and conduct feasibility study on its forensic application. Using 15 random oligonucleotide sequences as primers, a small amount of DNA was amplified with low specificity, and the amplified product was used as a template to perform specific locus amplification. Apoptosis was confirmed by PMCT118, GABAR, DYS19, D18S849, D3S1744, D12S1090, D8S1179, D2 1S11, D18S5 1, D5S818, D13S3 17, D7S82 0, D3S13 58, vWA, FGA, TH0 1, CSF1PO, TPOX, D16S539 and Amelogene PEP PCR at 20 loci was compared with direct PCR analysis. The typing results were consistent, and 1 ng DNA was correctly typed. PEP method can be used for forensic tests, is conducive to the use of trace evidence, the physical examination materials to provide as much information as possible