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目的:探讨二烯丙基二硫化物(DADS)对食管癌细胞株ECA-109体外侵袭能力的影响及其作用机制。方法:MTT法检测DADS对食管癌ECA-109细胞活力的影响,Transwell侵袭试验测定DADS在不同浓度时对ECA-109细胞侵袭能力的抑制作用。Western blot检测DADS在不同浓度下对ECA-109细胞MMP-2、MMP-9,TIMP-1、TIMP-2蛋白表达水平的影响。结果:DADS作用24h后,当浓度大于30μg/ml时,DADS可以显著抑制ECA-109细胞的活力,且随浓度升高其抑制作用相应增加;在浓度为10μg/ml-30μg/ml时,DADS能够明显减少穿过侵袭小室基底膜的肿瘤细胞数量,且抑制作用呈剂量依赖性;DADS可以使ECA-109细胞中MMP-2、MMP-9的表达水平降低,同时TIMP-1、TIMP-2的水平升高。结论:DADS在体外能够显著降低食管癌细胞的侵袭能力,其作用机制可能与DADS抑制MMP-2、MMP-9表达,上调TIMP-1、TIMP-2的表达有关。
Objective: To investigate the effect of diallyl disulfide (DADS) on invasiveness of esophageal carcinoma cell line ECA-109 in vitro and its mechanism. METHODS: The effect of DADS on the viability of ECA-109 cells was detected by MTT assay. The inhibitory effect of DADS on the invasiveness of ECA-109 cells was assayed by Transwell invasion assay. The effect of DADS on the protein expression of MMP-2, MMP-9, TIMP-1 and TIMP-2 in ECA-109 cells was detected by Western blot. RESULTS: After treated with DADS for 24 hours, DADS significantly inhibited the viability of ECA-109 cells when the concentration of DADS was more than 30μg / ml, and its inhibitory effect increased with the increase of DADS concentration. When DADS was 10μg / ml-30μg / ml, DADS could decrease the expression of MMP-2 and MMP-9 in ECA-109 cells, while the expression of TIMP-1, TIMP-2 The level of rise. CONCLUSION: DADS can significantly reduce the invasiveness of esophageal cancer cells in vitro. The mechanism may be related to the inhibition of the expression of MMP-2 and MMP-9 by DADS and the up-regulation of TIMP-1 and TIMP-2.