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本研究从全国各地收集到不同生态类型大粒材料36份,小粒材料12份。运用已经定位的分子标记共7对,经PCR扩增,用9%丙烯酰胺凝胶电泳,统计每个SSR位点,最后采用SPSS软件作方差分析。结果显示:选出3个与大粒相关的实用性标记,分别为GNE070、Satt126和Satt633。可以被一个或一个以上标记检出的大粒材料占35(大粒材料共36份),检出率高达97.2%。研究结果表明用GNE070、Satt126和Satt633联合检测,可以应用于分子标记辅助育种大粒育种实践。
In this study, 36 macadamia materials of different ecological types and 12 pellet materials were collected from all over the country. A total of 7 pairs of molecular markers have been mapped and amplified by PCR. Nine SSR sites were analyzed by 9% acrylamide gel electrophoresis. Finally, SPSS software was used for variance analysis. The results showed that three practical markers related to large grains were selected, namely GNE070, Satt126 and Satt633. Large particles of material that can be detected by one or more markers accounted for 35 (a total of 36 large particles of material), the detection rate as high as 97.2%. The results showed that the combined detection of GNE070, Satt126 and Satt633 could be applied to the molecular marker assisted breeding of large-grain breeding practice.