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利用大鼠4管道闭塞全脑缺血再灌注损伤模型,观察脑缺血后大鼠学习获得和记忆巩固能力的变化以及黄芪注射液的保护作用。结果显示,缺血组实验前经过和未经过训练的大鼠游完水迷宫全程的时间及错误次数均显著高于假手术组,黄芪组上述两项指标均小于缺血组。脑缺血30min再灌流7d,缺血组海马CA_1区大部分锥体细胞消失,残留的细胞存在明显的缺血改变,胶质细胞增生,CA_2、CA_3区基本正常,CA_4区散在锥体细胞坏死。黄芪组灌流7d CA_1区绝大多数细胞存活。提示全脑缺血引起了大鼠智能障碍,黄芪对缺血后动物行为和病理损害具有一定的保护作用。
The model of occlusion of global cerebral ischemia-reperfusion injury was established in rats with 4-channel occlusion. The learning and memory consolidation ability and the protective effect of Astragalus mongholicus injection in rats after cerebral ischemia were observed. The results showed that the time and number of errors in the complete maze of rats with and without training before ischemia were significantly higher in the ischemic group than those in the sham-operated group. The two indexes in the astragalus group were less than those in the ischemic group. Most of the pyramidal cells disappeared in ischemic hippocampal CA1 region, and the remaining cells showed obvious ischemic changes. The proliferation of glial cells was observed. The area of CA_2 and CA_3 were almost normal. The area of CA_4 was scattered in pyramidal cell necrosis . Astragalus group CA7 area 7d most of the cells survive. It is suggested that global cerebral ischemia caused the rats’ mental retardation. Astragalus membranaceus could protect the behavior and pathological damage of the animals after ischemia.