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为明确福建省烟粉虱种群遗传结构特征,基于福建省烟粉虱不同地理种群中40个代表性的线粒体COI基因序列,分析了种群遗传多样性、遗传分化及分子变异情况,并构建了单倍型系统发育树与网络图。结果显示:在590 bp长度的mt COI基因序列中有效位点558个,其中187个核苷酸位点存在变异;序列核苷酸中A、T、C、G含量分别为42.32%、24.36%、20.25%、13.06%,其中A+T的含量为66.68%,表现出明显的A+T偏向性;共检测出11个单倍型,其中Hap3、4、7、9、11为特殊单倍型;种群多样性指数为0.838,核苷酸多样性指数为0.093,表明遗传多样性水平较高;AMOVA分析表明种群遗传变异主要来自种群内,总种群遗传分化系数仅为0.027,种群遗传分化较低。表明福建烟粉虱种群基因交流未受地理距离明显影响,种群遗传分化不显著。
In order to understand the genetic structure of Bemisia tabaci (Gennadius) population in Fujian Province, based on 40 representative mitochondrial COI gene sequences among different geographic populations of Bemisia tabaci (Gennadius), the genetic diversity, genetic differentiation and molecular variation of Bemisia tabaci were analyzed. Dominant phylogenetic tree and network map. The results showed that there were 558 valid mt COI gene sequences in 590 bp, of which 187 nucleotide sites were variable. The contents of A, T, C and G in nucleotide sequence were 42.32% and 24.36% , 20.25% and 13.06%, respectively. The content of A + T was 66.68%, which showed obvious A + T bias. A total of 11 haplotypes were detected, of which Hap3, 4, The population diversity index was 0.838 and the nucleotide diversity index was 0.093, indicating a high level of genetic diversity. The analysis of AMOVA indicated that the genetic variation of population mainly came from the population. The genetic differentiation coefficient of the total population was only 0.027. low. The results showed that the gene exchange of B. tabaci population in Fujian was not significantly affected by the geographical distance, and the genetic differentiation was not significant.