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[目的]对卑霉素产生菌Tü-57进行诱变的育种。[方法]先对4种诱变方式的致死剂量进行筛选,再采用复合诱变的方法,用紫外线和微波2种诱变剂与链霉素和氯化锂2个底物标记物,两两结合作用于原始菌,得到诱变株,并测定其卑霉素产量。[结果]获得了一株卑霉素高产菌株ul36,其作用条件为紫外350 s,链霉素0.8μg/ml,与原始菌相比,ul36卑霉素产量提高2.58倍。诱变株经多次发酵测效价结果稳定。[结论]为将卑霉素应用于实际生产奠定了基础。
[Objective] Breeding of mutagenesis of B-producing strain Tü-57 was carried out. [Method] The lethal doses of four kinds of mutagenesis methods were screened first, and then the mutagens of two kinds of mutagens of UV and microwave were used with two substrate markers of streptomycin and lithium chloride. Combined with the original bacteria, mutagenized strains were obtained, and the determination of the yield of bixin. [Result] The result showed that the yield of ul36, a strain of pluriprin, was 350 uv and 0.8 ug / ml. The yield of ul36 was 2.58 fold higher than that of original strain. The mutagenesis strain was stable after multiple fermentations. [Conclusion] This laid the foundation for the application of binomile in practical production.