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运用PCR-DGGE技术对我国准格尔露天煤矿不同复垦时间土壤细菌组成及其多样性变化进行了研究,结果表明:1)复垦区的土壤细菌主要是厚壁菌门、拟杆菌门、放线菌门、变形菌门、酸杆菌门、芽单胞菌门和未知细菌类7类,其中,放线菌门、拟杆菌门和变形菌门为优势类群,占到全部细菌的81.2%;2)成功完成矿山土壤样品微生物总DNA提取和PCR-DGGE的分析检测,在69个测序序列中,不可培养细菌占到总数的62.3%;3)各复垦阶段存在共有细菌类群,但更多的细菌类群是随复垦时间不同而发生变化的。4)豆科-禾本科牧草种植区的土壤细菌多样性指数要高于乔灌草类型,这可能与豆科植物中丰富的根瘤菌利于土壤细菌生长发育有关。
The results showed that: 1) The soil bacteria in the reclamation area were mainly Firmicutes, Bacteroidetes, Actinomycetes, Bacillus acidophilus, Bacillus monilia and unknown bacteria 7 categories, of which, actinomycetes, Bacteroidetes and Proteobacteria as the dominant group, accounting for 81.2% of all bacteria ; 2) The successful completion of microbial total DNA extraction and PCR-DGGE analysis of soil samples from mines, 62.3% of the total number of uncultured bacteria in 69 sequencing sequences; 3) There are common bacterial groups in each reclamation stage, but more More bacterial groups are different with reclamation time changes. 4) The diversity index of soil bacteria in leguminous-pastoral areas was higher than that of trees and shrubs, which may be related to the enrichment of Rhizobia in legumes for soil bacterial growth and development.