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目的了解葛根素注射液(葛根素)在大鼠急性肺损伤(ALI)中的对CRP的影响。方法将实验动物随机按时间分为8组(并行油酸+葛根素)即:治疗A组:6h组;治疗B组:8h组;治疗C组:12h组;治疗D组:(行油酸+葛根素后12h再追加葛根素)16h组。用生理盐水替换葛根素为油酸损伤对照组,分别为A′、B′、C′、D′组。用检测谷光甘肽(GSH)和谷光甘肽过氧化物酶(GSH-PX)来评定氧自由基水平;用CRP评定急性炎性反应程度。结果①CRP:治疗组间有明显差异;治疗A组水平最高,C组最低;治疗A组明显高于对照A′组;治疗C、D组明显低于对照组;②GSH:治疗组组间、对照组组间有明显差异;治疗组A组明显高于对照组;③GSH-PX:治疗组组间、对照组组间有明显差异;治疗A、C组明显高于对照组。结论葛根素注射液是一种可用的抗氧自由基药物,但起效较慢;可升高CRP浓度,但维持时间较短;建议重复给药时间不超过6～8h。
Objective To investigate the effect of puerarin injection (puerarin) on CRP in acute lung injury (ALI) in rats. Methods The experimental animals were randomly divided into 8 groups (concurrent oleic acid + puerarin): treatment A group: 6 h group; treatment B group: 8 h group; treatment C group: 12 h group; treatment D group: (line oleic acid + Puerarin was added to the 16h group 12 h after puerarin. The puerarin was replaced by physiological saline as the oleic acid control group, which was A’, B’, C’, and D’ groups, respectively. Oxygen free radicals levels were assessed with the detection of glutathione (GSH) and glutathione peroxidase (GSH-PX); the extent of acute inflammatory reactions was assessed with CRP. Results 1CRP: There was a significant difference between the treatment groups; the treatment A group had the highest level and the C group had the lowest; the treatment A group was significantly higher than the control A′ group; the treatment C, D group was significantly lower than the control group; 2GSH: the treatment group, the control group There were significant differences between the groups; treatment group A group was significantly higher than the control group; 3GSH-PX: There was a significant difference between the treatment group and the control group; treatment A, C group was significantly higher than the control group. Conclusion Puerarin injection is a kind of available anti-oxygen free drug, but its effect is slower; it can increase the concentration of CRP, but the maintenance time is short; it is recommended that the repeated administration time should not exceed 6-8 hours.