采用反相高效液相色谱外标法,以乙腈-水作为流动相,用Kromasil ODS柱和紫外检测器(205nm)研究了测定硅氟唑含量的方法,并探讨了测定条件。结果表明,方法的标准偏差0.50%,变异系数0.52%,加标回收率100.17%;硅氟唑进样量2.5～10.0μg时,方程呈良好的线性关系。方法简便、快速,可满足定量分析的需要。 The method of determining the content of silicon tetrazolium was studied by reversed-phase high-performance liquid chromatography with acetonitrile-water as the mobile phase and the Kromasil ODS column and UV detector (205 nm). The determination conditions were also discussed. The results showed that the standard deviation of the method was 0.50%, the coefficient of variation was 0.52%, and the recovery rate was 100.17%. The equation showed a good linearity when the injection volume of simeconazole was 2.5 ~ 10.0μg. The method is simple, rapid and can meet the needs of quantitative analysis.