【摘 要】
：
Decades of biochemical studies have advanced DNA beyond its primary role as genetic blueprint.DNAzymes are single-stranded enzymatic DNA molecules that do not exist in nature.They are ideal candidates for gene silencing owing to their scalability by solid
【机 构】
：
Neuroscience Program,Department of Neurology,Houston Methodist Research Institute,Houston,TX,USA
论文部分内容阅读
Decades of biochemical studies have advanced DNA beyond its primary role as genetic blueprint.DNAzymes are single-stranded enzymatic DNA molecules that do not exist in nature.They are ideal candidates for gene silencing owing to their scalability by solid-phase synthesis (without batch variations),reprogrammability by directed evolution and local sequence alterations,compatibility with diverse delivery methods,and capability of achieving high catalytic turnover independent of any auxiliary proteins.With these unique features,various artificially evolved DNAzymes have been employed as theranostic tools in designing biosensors and logic gates,RNA/DNA cleavage and ligation,phosphorylation and dephosphorylation,DNA photorepair,and peptide side-chain modifications,to name but a few (Ponce-Salvatierra et al.,2021).This perspective will focus on the functional aspects and therapeutic potentials of RNA-cleaving DNAzymes.
其他文献
The association between traumatic brain injury (TBI) and an increased risk of neurodegeneration has been recognized for some decades now (Faden and Loane,2015),with recent evidence suggesting that a history of TBI,either as repeated concussive/mild TBI in
Introduction:In the 1680s,Antonie van Leewenhoek was the first to observe the differences in the human gut microbiome versus the oral cavity diversity,and it led to the birth of a new term,“microbiota”.
In a symbiotic and mutualistic manner,the gut microbiota strongly influences many vital aspects of host physiology,biology,repair,and regeneration.The role of microbiota in many areas of regenerative medicine is just beginning to emerge,but it is under-re
Alzheimer\'s disease (AD) is a neurodegenerative disease that is characterized by an age-dependent progressive decline of memory,impairment of cognitive functions and changes in personality and behavior.Despite the improvement in understanding of the me
Childhood maltreatment (CM) has been consistently linked with numerous detrimental outcomes concerning physical and psychological health.However,few studies have explored vulnerability to neurological disorders after CM.Early life adversity,in the form of
Late stage blindness and visual impairment (BVl) affects over 400 million adults worldwide.These disabilities severely impact the ability of adults to function independently,reduce their quality of life,and worsen socio-economic burdens on health care sys
Alzheimer\'s disease (AD) is the most common cause of dementia in the elderly.Unfortunately,there are no effective therapies currently available for prevention and treatment of AD.As it is clear now,the etiology of AD is multifactorial and complex.This
A recent study by McDonald et al.(2021) focused on how peripheral glucose metabolism and handling are compromised in amyotrophic lateral sclerosis (ALS).Dysfunctions in glucose and energy metabolism have been identified in transgenic mouse models and pati
The mammalian central nervous system(CNS) is highly complex,with a vast array of processes and interactions occurring in a dynamic and often transient manner.How these processes are combined to regulate our behavior remains poorly understood.This has in t
Herein,the rationale and supporting evidence for the promise of developing K+/Cl-co-transporter-2 (KCC2) neuromodulatory therapies for spinal cord injury (SCl) is discussed.SCI is commonly a life-changing,unforeseen neurotrauma that has devastating conseq