【摘 要】
：
Dengue fever,caused by dengue viruses (DENVs),is a widespread mosquito-borne zoonotic disease;however,there is no available anti-dengue vaccine for worldwide use.In the current study,a DNA vaccine candidate (pV-D4ME) expressing prM-E protein of DENV serot
【机 构】
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Department of Microbiology, School of Basic Medical Sciences, Capital Medical University, Beijing 10
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Dengue fever,caused by dengue viruses (DENVs),is a widespread mosquito-borne zoonotic disease;however,there is no available anti-dengue vaccine for worldwide use.In the current study,a DNA vaccine candidate (pV-D4ME) expressing prM-E protein of DENV serotype 4 (DENV-4) was constructed,and its immunogenicity and protection were evaluated in immunocompetent BALB/c mice.The pV-D4ME candidate vaccine induced effective humoral and cellular immunity of mice against DENV-4 in vivo when administered both at 50 μg and 5 μg through electroporation.Two weeks after receiving three immunizations,both doses of pV-D4ME DNA were shown to confer effective protection against lethal DENV-4 challenge.Notably,at 6 months after the three immunizations,50 μg,but not 5 μg,of pV-D4ME could provide stable protection (100％ survival rate) against DENV-4 lethal challenge without any obvious clinical signs.These results suggest that immunization with 50 μg pV-D4ME through electroporation could confer effective and long-term protection against DENV-4,offering a promising approach for development of a novel DNA vaccine against DENVs.
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