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目的:观察蝎毒多肽提取物(PESV)对非小细胞肺癌细胞株A549的增殖抑制作用及可能的作用机制。方法:采用噻唑蓝(MTT)法观察不同浓度PESV对A549细胞生长与增殖的影响,下游实验将对数生长期的A549细胞分为阴性对照组、PESV低、中、高剂量组,应用流式细胞术、免疫细胞化学法、Western blot法检测PESV干预后细胞周期及VEGF,HIF-1α和PTEN蛋白表达的变化。结果:MTT结果显示,PESV在一定浓度范围内对A549细胞的增殖活性有明显抑制作用(P<0.01)。流式细胞法、免疫细胞化学法及Western blot法结果显示,PESV干预后能使A549细胞阻滞于G0/G1期,并显著下调HIF-1α,VEGF表达,上调PTEN表达。结论:PESV能够抑制A549细胞的增殖,其作用机制可能与影响血管生成因子VEGF,HIF-1α和PTEN的表达而直接抑制细胞增殖、阻滞细胞周期和抑制血管生成有关。
Objective: To observe the inhibitory effect of PESV on the proliferation of non-small cell lung cancer cell line A549 and its possible mechanism. Methods: MTT assay was used to observe the effect of different concentrations of PESV on the growth and proliferation of A549 cells. A549 cells were divided into negative control group, low, middle and high dose PESV group, Cell cycle, immunocytochemistry and Western blot were used to detect the changes of cell cycle and the expression of VEGF, HIF-1α and PTEN protein after PESV intervention. Results: MTT results showed that PESV significantly inhibited the proliferation of A549 cells in a certain concentration range (P <0.01). The results of flow cytometry, immunocytochemistry and Western blot showed that PESV could block A549 cells arrest in G0 / G1 phase and down-regulate the expression of HIF-1α and VEGF and up-regulate the expression of PTEN. CONCLUSION: PESV can inhibit the proliferation of A549 cells. Its mechanism may be related to the direct inhibition of cell proliferation, cell cycle arrest and angiogenesis by affecting the expression of angiogenic factors VEGF, HIF-1α and PTEN.