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研究了腈菌唑导致的细胞膜穿孔对离体培养斜纹夜蛾Spodoptera litura卵巢细胞(SL细胞)膜通透性的影响。结果表明:腈菌唑可使不能穿过SL细胞膜的大分子物质荧光染料PI大量穿过细胞膜进入胞内,20和40μg/mL腈菌唑处理后12 h,SL细胞荧光强度增加率分别为11.95%和25.80%;处理后24 h,SL细胞荧光强度增加率分别为27.77%和57.490%。腈菌唑也可明显提高SL细胞内大分子物质乳酸脱氢酶的漏出率,20和40μg/mL腈菌唑处理SL细胞24 h后,胞内乳酸脱氢酶漏出率分别为30.66%和43.93%;处理后48 h漏出率分别为41.22%和57.91%。腈菌唑可以明显提高SL细胞胞内钙离子含量,20和40μg/mL腈菌唑处理SL细胞24和48 h,胞内钙荧光强度与对照差异显著。处理后24 h,腈菌唑对SL细胞的LC_(50)值为35.84μg/mL,明显高于典型细胞毒剂鱼藤酮的活性。当质量比为1:1和2:1时,腈菌唑与鱼藤酮联用对SL细胞处理后24 h的LC_(50)值为43.92和26.09μg/mL,共毒系数分别为137.60和188.49,增效作用显著,随着腈菌唑的含量增加,增效作用增加。腈菌唑对SL细胞具有良好的抑制作用,可以打通限制物质穿透的细胞膜屏障,提高农药活性成分的有效利用率。
The effect of myclobutanil-induced cell membrane perforation on membrane permeability of Spodoptera litura ovarian cells (SL cells) cultured in vitro was investigated. The results showed that myclobutanil could penetrate the membrane of macromolecular fluorescent dye PI, which could not cross the membrane of SL cell, into the cells. After 12 and 20 μg / mL of myclobutanil, the increase of fluorescence intensity of SL cells was 11.95 % And 25.80% respectively. After 24 h treatment, the increase rate of SL cell fluorescence intensity was 27.77% and 57.490% respectively. Myclobutanil could also significantly increase the leakage rate of lactate dehydrogenase (LDH) in the SL cell. After 24 and 24 h treatment with 20 and 40 μg / mL myclobutanil, the leakage rates of intracellular lactate dehydrogenase were 30.66% and 43.93 %; 48 h after treatment leakage rates were 41.22% and 57.91%. Myclobutanil significantly increased the intracellular calcium content of SL cells. The intracellular calcium fluorescence intensity of SL cells treated with 20 and 40 μg / mL myclobutanil for 24 and 48 h was significantly different from that of the control. The LC 50 value of myclobutanil to SL cells was 35.84μg / mL 24 h after treatment, which was significantly higher than that of the typical cytotoxic agent rotenone. When the mass ratio of 1: 1 and 2: 1, the LC 50 value of myclobutanil combined with rotenone for 24 h after treatment of SL cells were 43.92 and 26.09 μg / mL, and the co-toxicity coefficients were 137.60 and 188.49, respectively. Synergism is significant, with the increase of myclobutanil, synergism increased. Myclobutanil has a good inhibitory effect on SL cells, which can open up the cell membrane barrier that limits the material penetration and improve the effective utilization rate of active ingredients of pesticides.