论文部分内容阅读
研究水稻少分蘖高秆突变体的分子机理,鉴定出新的水稻少分蘖高秆基因。本研究利用γ射线辐射诱变籼稻品种泸恢H103,获得一个少分蘖高秆突变体,命名为ltn1(low-tiller number)。研究了该突变的主要农艺性状与遗传方式,并对突变体基因LTN1进行了分子定位。结果显示突变体的株高,有效穗,结实率,千粒重,单株产量均与野生型存在显著差异。遗传分析表明突变体ltn1受一对隐性基因控制,将突变体和沈农265的F2代中的突变型个体作为定位群体,利用SSR和Indel分子标记将突变体定位在水稻第8染色体标记RM3840和RM23611之间,物理距离为135 kb的区间。在这个区间内有个预测注释基因LOC_Os08g44510。
The molecular mechanism of rice tillering high-stalk mutant was studied and a new rice tillering high-stalk gene was identified. In this study, γ-ray irradiation was used to mutagenize Luhui H103, a tiller-less mutant named ltn1 (low-tiller number). The main agronomic traits and inheritance of this mutation were studied, and the mutant gene LTN1 was molecularly mapped. The results showed that there were significant differences in the plant height, effective panicle, seed setting rate, 1000-grain weight and yield per plant of the mutant. Genetic analysis showed that the mutant ltn1 was controlled by a pair of recessive genes. The mutant and Shennong 265 F2 were used as loci. The SSR and Indel molecular markers were used to locate the mutant on the chromosome 8 RM3840 And RM23611, with a physical distance of 135 kb. There is a predictive annotation gene LOC_Os08g44510 in this interval.