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我们建立的K562/ADM耐药细胞株,在ADM浓度为2.4μg/m1(4.46μM)中已稳定培养3.5个月,传了30—35代,K562/ADM亦具有多药耐受件(Multidrug Resistance,MDR)的特点,对ADM、VCR、AT—1258和DDP的耐受性分别为K562的114.7、94.0、13.3和7.4倍,但对5—FU不产生交叉耐药。K562和K562/ADM的倍增时间分别为19.2h和52.8h,集落生成率分别为37.5％和11.1％,K562染色体数为34—68,中位数为56;K562/MDM染色体数为32—90,中位数为50,K562/ADM可做为耐药机理和克服耐药措施研究的极好模型。
Our established K562/ADM drug resistant cell line has been stably cultured for 3.5 months at an ADM concentration of 2.4 μg/m1 (4.46 μM) and has been transmitted for 30-35 passages. K562/ADM also has a multidrug resistant member (Multidrug) The characteristics of Resistance, MDR), to ADM, VCR, AT-1258, and DDP were 114.7, 94.0, 13.3, and 7.4 times that of K562, respectively, but did not produce cross-resistance to 5-FU. The doubling times of K562 and K562/ADM were 19.2 h and 52.8 h, the colony generation rate was 37.5% and 11.1%, the K562 chromosome number was 34-68, and the median was 56; the K562/MDM chromosome number was 32-90. With a median of 50, K562/ADM can be used as an excellent model for drug resistance mechanisms and overcoming drug resistance measures.